218k views
0 votes
You need to determine the sequence of a linear decapeptide. The following experiments have been performed:

1. Edman degradation generates 10 amino acids: Q, E, M, R, K, C, F, P, Y, A.
2. Cyanogen bromide (CNBr) cleavage gives two pentapeptides (5 aa’s).
3. Trypsin cleavage gives two tripeptides (AMR and QFK), and a tetrapeptide.
4. Chymotrypsin cleavage gives a dipeptide QF, a single amino acid, and a heptapeptide (7).
5. The C-terminal residue is identified as Cys using carboxypeptidase C, which removes amino acids from the C-terminal end of a polypeptide.
Give two possible sequences for the decapeptide, using aa three-letter codes.

User Mythics
by
8.3k points

1 Answer

5 votes

Answer:

The two possible sequences for the decapeptide using a three-letter codes

are :

ii) Q F K A M R P E Y C

II) Q F K A M R E P Y C

Step-by-step explanation:

The two possible sequences for the decapeptide using a three-letter codes

are :

ii) Q F K A M R P E Y C

II) Q F K A M R E P Y C

This possible sequences are gotten from Edman degradation , Cyanogen bromide and Trypsin digestion and also Chymotrypsin digestion. also note that the codons used to code these amino acids can also be written in a sequence to obtain a three code sequence

User Todd DeLand
by
7.5k points
Welcome to QAmmunity.org, where you can ask questions and receive answers from other members of our community.

9.4m questions

12.2m answers

Categories