Question

In studying a particular biomolecule (a protein, nucleic acid, carbohydrate, or lipid) in the laboratory, the biochemist first needs to separate it from other biomolecules in the sample (that is, it needs to be purified). Specific purification techniques are described later in the text. However, by looking at the monomeric subunits and functional groups of a biomolecule, you should have some ideas about the characteristics of the molecule that would allow you to separate it from other molecules. Look for differences in ionizable groups, polarity, solubility in water, size, and shape. Based on the characteristics of each type of molecule, select the way(s) in which amino sugars such as glucosamine could be separated from steroid hormones such as estradiol

Answer 1

Sugars can be separated from steroidal homones by observing the solubility of these two molecules in water and observing the presence of a negatively altered group in one of the two molecules.

Step-by-step explanation:

Steroid hormones are molecules derived from cholesterol and usually present a negative change in their structure, which differs from sugars such as carbohydrates and allows these two molecules to be purified, being isolated from each other.

Another way of purifying these two molecules is to observe the solubility of both in water, since sugars are completely soluble in water, without altering the osmotic balance of the cells, however, the same cannot be observed with steroid hormones.