Answer:
The French establishment was wiped out by the Spanish in 1565. With the capture of Fort Caroline, Huguenots either fled into the wild mainland or were killed in the subsequent massacre at Matanzas Inlet.
In 1568, Dominique de Gourgue further explored the area, and, with the help of his allies the Saturiwa Indians, massacred the Spanish garrison in retaliation, but he did not capitalize on this action.