Answer:
This question is incomplete
Step-by-step explanation:
Introns are non-coding regions of a DNA that removed by RNA splicing prior to translation. Alignment is usually done between sequences to see and understand the identity and similarity between two or more sequences.
A region/base is said to be conserved if there is NO change in any base in that particular region. A multiple sequence alignment (MSA) can be used to align the donor sites of all the introns to see the bases that have not "changed" (and still remained in there exact position) hence conserved across all the donor sites.
NOTE: The donor site of an intron is the 5' end, thus the first five bases in the 5' end are to be used here