Answer:
DNA gyrase
Step-by-step explanation:
DNA gyrase is a bacterial enzyme that plays an important role in catalyzing the negative supercoiling of circular chromosomal DNA. This enzyme belongs to a class of enzymes called topoisomerases which play a major role in transitioning of DNA structure. DNA gyrase conversion of covalently closed circular DNA to supercoiled DNA is ATP-dependent.
The importance of DNA gyrase cannot be overemphasized, because for DNA synthesis to occur, the DNA must have a single-stranded template, hence, DNA gyrase play an important role in unwinding the double-stranded DNA for replication. It has the ability to introduce negative supercoils and relax positive supercoils prior to replication.