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When looking at the effect of PH on sucrase activity, what was the wavelength of light used in the spectrophotometer?

2 Answers

3 votes
540 mn when I calculate it into the computer
User Thomas Dignan
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7 votes

Answer:

540 nm

Step-by-step explanation:

Sucrase is a digestive enzyme. It is the catalyst in the hydrolysis of sucrose into the two subunits that it is made up of namely; fructose and glucose.

Enzymes are known to function best at optimum pH. As the pH of sucrase departs from its ideal pH, its activity decreases.

The activity of sucrose is studied using a spectrophotometer of wavelength about 540nm.

User CuriousDawg
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