Question

The polymerase chain reaction, or PCR, is a tool used frequently in biotechnology. Because PCR mimics the natural DNA replication that occurs in the cell, the two processes share many characteristics. Sort the descriptions of these processes by dragging them to the appropriate boxes. The descriptions may apply to natural replication only, to PCR only, or to both processes.

Answer 1

The polymerase chain reaction (PCR) is a technique used to rapidly increase the number of copies of specific regions of DNA for further analysis. PCR mimics the natural DNA replication that occurs in the cell and involves denaturing, annealing, and extension steps. DNA polymerase and primers are used in both natural replication and PCR processes to initiate replication and add nucleotides to the DNA strand.

Step-by-step explanation:

The polymerase chain reaction (PCR) is a technique used to rapidly increase the number of copies of specific regions of DNA for further analysis. PCR involves three steps: denaturing, annealing, and extension. Denaturing involves heating DNA to break the bonds holding the two DNA strands together, while annealing involves cooling the single strands of DNA and mixing them with short DNA segments called primers. Primers have base sequences that are complementary to segments of the single DNA strands, allowing bonds to form between the DNA strands and primers.

PCR mimics the natural DNA replication that occurs in the cell. Both natural replication and PCR require the use of DNA polymerase and primers to initiate replication and add nucleotides to the DNA strand. Howevere, in the case of natural replication, primers composed of RNA are used, while in PCR, DNA primers are used for their stability. PCR is a powerful technique in molecular biology with various applications in research, forensic, and clinical laboratories.

Answer 2

PCR

Produces million of copies of a segments of DNA and

Requires cycles of temperature.

PCR.

When large number of copies of a particular gene fragment is needed from smallest quantity of DNA molecules,the techniques of genetic engineering usually employed is called PCR. It involves denaturation of template DNA,the use of Primers(20 base pairs),the use of enzyme DNA polymerase for building of new strands of DNA.

Natural Replication.

produces a copy of the whole genome

It occurs before mitosis and meiosis

Begins at the origin of replication.

Both.

Requires primers

Requires DNA polymerase

Results in Semi conservative replication

Step-by-step explanation:

When rapid production of large copies of particular DNA fragments is needed from a small molecules of fragment of the original DNA fragments is needed the tool of genetic engineering used is called PCR.

It involves denaturation of the template DNA at 95 degrees for separation to single strands,

the attachments of primers(20 base pairs of complementary bases to the template DNA) at 65 degree a process called annealing and the use of polymerase enzyme for building on chains of DNA , during elongation.

The enzyme are extracted from thermophilic organisms,therefore could withstand the high temperature.The polymerization can proceed for a continuous cycle with million of new DNA chains produced by the polymerization Chain machine