Question

The authors state in the general procedures that the reaction was monitored by TLC. How would this be done? What would you spot in each lane? How would you know the reaction was done?

Answer 1

Thin Layer Chromatography (TLC) can be used to analyze chemical reactions. During this reaction monitoring, a typical TLC plate would have three spots: the reactant lane, the reaction mixture lane, and a "co-spot" where reaction product would be spotted directly on top of reactant.

The co-spot serves as a reference point and is vital for reactions where reactant and product have similar Rfs, and many other variations of eluent tracking.

To indicate completion of the reaction, the disappearance of a spot (usually the starting reactant) is observed.