Answer:
By using specific primers for PCR amplification and DNA sequencing
Step-by-step explanation:
In molecular biology, different techniques are used to clone the full sequence of desired genes. In general, gene cloning is based on the assembly of genetically engineered recombinant DNA cloning vectors into host organisms. The cloning procedure generally consists of isolating target DNA sequences to be cloned, ligation into a corresponding vector, transfection and screening of clones. The recombinant DNA is first amplified by PCR. Subsequently, DNA is sequenced and finally assembled by bioinformatic methods in order to characterize the full sequence of the gene.