Determine the correct sequence of events during the creation of recombinant DNA.
I. Plasmid is cut open with enzymes
II. Target gene is cut out with enzymes
III. Plasmid is inserted into the bacterium
IV. Target gene is bonded to the plasmid with Ligase
A. IV, I, II, III
B. II, I, IV, III
C. I, II, III, IV
D. IV, III, I, II