Question

QUESTION 31 Chipmunk mitochondrial DNA was fingerprinted using the single primer amplified region technique (SPAR) and the PCR reaction. One four base primer was used. If the DNA from chipmunk mitochondria is a circlular piece of DNA about 16000 base pairs long, how many fragments should be produced by this technique

Answer 1

It depends on the size of the amplified DNA fragments (for example for SPAR markers consisting of 200 bp in length >> 1600 / 200 = 8)

Explanation:

The PCR based single primer amplification reaction (SPAR) methods are tools for detecting genetic diversity by using DNA markers such as Simple Sequence Repeats (ISSRs), Minisatellite DNA regions (DAMDs) and Random Amplified Polymorphic DNAs (RAPDs), which can be used alone or combined with each other. In the last years, the SPAR methods have gained attention in the scientific community because they are cost-effective and highly effective for the detection of both intraspecific and interspecific genetic variation. In the SPAR methods, the size of the bands will depend on the length of the amplified DNA fragments.