Question

o which plates do you compare in order to detect the activity of the ligase? Explain. Can you tell, based on the results, whether the ligase enzyme worked?

Answer 1

Tube no. 6 in which 0.2 ml plated with no dilution

Step-by-step explanation:

Tube no. 6 in which 0.2 ml plated with no dilution is used has higher the activity of ligase enzymes as compared to other tubes. The ligase enzyme worked as to speedup the binding of two molecules and making one large molecules. In Tube no. 6, 320 colonies were formed which is the highest number of colonies as compared to others so we can say that on Tube no. 6 ligase activity is higher as compared to others.