Question

A DNA sequence encoding a five-amino acid polypeptide is given below. …ACGGCAAGATCCCACCCTAATCAGACCGTACCATTCACCTCCT…

TGCCGTTCTAGGGTGGGATTAGTCTGGCATGGTAAGTGGAGGA…
a. Locate the sequence encoding the five amino acids of the polypeptide and identify the template and coding strands of DNA.
b. Give the sequence and polarity of the mRNA encoding the polypeptide.
c. Give the polypeptide sequence and identify the N-terminus and C-terminus.
d. Assuming the sequence above is a bacterial gene, identify the region encoding the Shine-Delgarno sequence.
e. What is the function of the Shine-Delgarno sequence?

Answer 1

Step-by-step explanation:

a. The template strand is:

ACGGCAAGATCCCACCCTAATCAGACCGTACCATTCACCTCCT

The coding strand is

TGCCGTTCTAGGGTGGGATTAGTCTGGCATGGTAAGTGGAGGA

The sequence encoding the five amino acids is: 3' CTA-ATC-AGA-CCG-TAC-CAT 5'

b. 5' AUG-GUA-CGG-UCU-GAU-UAG 3'

c. N terminus Met-Val-Arg-Ser-Asp C terminus

d. GGAGGA

e. The shine Delgarno sequence as a mRNA binding site for mRNA's binding to the small subunit ribosome.