Question

If a researcher has a DNA sample with a concentration of 0.6 micrograms per microliter, how many microliters of DNA must be transfered to conduct a 50 microliter reaction if the final concentration should be 3.9 nanograms per microliter

Answer 1

0.325 microliters

Step-by-step explanation:

1 microgram = 1000 nanograms

DNA sample concentration= 1000 X 0.6 = 600 nanograms / microliters

We need a total of 50 microliter reactions, and the concentration should be 3.9 nanograms per microliter.

So, we need total 50 X 3.9 = 195 nanograms in 50 microliters.

Therefore, it required 195 / 600 = 0.325 microliters of DNA sample to be transferred.