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Which is a method of making many copies of DNA?

O A. Nuclear transfer
B. Gel electrophoresis
C. Reverse transcriptase
D. Polymerase chain reaction

User Klor
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Answer:

D. Polymerase chain reaction

Step-by-step explanation:

PCR is very percise; used to amplify or copy a specific DNA target from a mixture of DNA molecules by:

  • two short DNA sequences (primers) are bind to start and end the DNA target
  • perform the PCR; DNA templates is added to a tube that contains primers, free nucleotides, and the enzyme (DNA polymearse)
  • it is mixed in a PCR machine that increases and decreases the temperature of the sample
  • mixture is heated to seperate the double strnaded DNA templated into single strands
  • mixture is cooled so the primers can bind to the DNA template
  • the DNA polymerase will synthesize to new strands of DNA from the primers
  • during synthesis, the end of the first cycle, the DNA molecule will consist of a new and old DNA strand
  • PCR continues with added cycles to repeat the steps
  • newly synthesized DNA segements serve as templates in later cycles; which can be copied over and over.
User Mark Nunberg
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