Answer:
The correct answer is "proteins are extracted from an environment, fragmented, and adaptors are added".
Step-by-step explanation:
The missing options of this question are:
A) Software aligns sequences and assemble large portions of genome.
B) The samples are subject to polymerase chain reaction for amplification.
C) The sequences of the amplified fragments are determined by next-generation methods.
D) Proteins are extracted from an environment, fragmented, and adaptors are added
The correct answer is option D) Proteins are extracted from an environment, fragmented, and adaptors are added.
Metagenomic sequencing refers to the amplification and determination of all nucleotide sequences in a sample, which might contain multiple microorganisms and viruses in order to understand the presence and proportions of microbiota. Metagenomic sequencing involves the use of next-generation methods, to amplify and characterize all the nucleotides sequences. It is false to affirm that the proteins are extracted directly from the environment in this technique, since metagenomic sequencing is focused on the nucleotide sequences.