A. Describe how C. parvum obtains the glucose it needs for glycolysis after it has infected another cell.
B. Explain the role of lactate dehydrogenase in enabling C. parvum to continue producing ATP by glycolysis.
C. Identify the independent variable used in the experiment.
D. Identify the difference between the control cells and the experimental cells used in the experiment.
E. Justify the use of a control in this experiment.
F. Justify the researchers using a different range of concentrations for FX11 than was used for gossypol.
G. Based on the data in Figure 1, Identify the concentration of gossypol that reduced C. parvum growth to 50% of that in control cells.
H. Researchers discovered a strain of C. parvum that expresses a functional variation of the lactate dehydrogenase gene. A DNA sequence comparison showed that the variant differs from the normal sequence in the region that codes for the enzyme’s allosteric site. Predict the effect of FX11 treatment on C. parvum that express this variant of lactase dehydrogenase.
I. Provide reasoning to support your prediction.
J. Explain how gossypol and FX11 might be used as drugs to treat C. parvum infections in humans without negatively affecting human cells.