Question

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A scientist wants to insert a human gene into bacteria, but she accidentally uses a different restriction enzyme on the human gene than she did on the plasma. What is most likely to occur? *


A) The plasmid would not contain the human gene because the cuts are not compatible.

B) The bacteria will be unable to host the plasmid.

C) The restriction enzyme will be unable to cut the human DNA

D) The bacterial DNA will not be cut by the restriction enzymes.

Answer 1

The correct answer is option A) "The plasmid would not contain the human gene because the cuts are not compatible".

Step-by-step explanation:

One of the basis of genetic engineering is to use the proper restriction enzymes to cut fragments of DNA. After cutting DNA with restriction enzymes most of the time the fragments have sticky ends, which are a few nucleotides that are one stranded and complementary to each other. Scientists take advantage of this characteristic and use the same restriction enzyme to cut genes and plasmid and later put it together with DNA ligases. If genes and plasmids are accidentally cut with different restriction enzyme it is very likely that the plasmid would not contain the human gene because the cuts are not compatible, since no sticky ends are generated.