Question

82. Your research team has recently identified a new gene and named short arm that when mutated causes the limb bud to cease growing shortly after the ZPA AER feedback loop is normally established. You hypothesize that this new gene acts downstream of BMPs to inhibit FGFs in the AER. Design an experiment or set of experiments that would test if this hypothesis is correct. Include what results would support or disprove the hypothesis.

Answer 1

The creation of the limbs is carried out by AER and ZVP. AER stands for apical ectodermal ridge and ZVP stands for polarising activity line.

Explanation:

• AER is responsible for the developmental progression in the distal to the anterior region and the ZVP is liable for the progressive advancement in the posterior to a backward region.

• AER and ZVP are interconnected on one another in the secretion of a sonic hedgehog, which eventually assists throughout the hox speech to promote the growth of the arm.

• The hypothesis that the new short-arm gene inhibits the effect of FGF (fibroblast growth factors) on AER can be tested and use a template of cultured cells.

• The AER may be disrupted in the model and the impact of the gene encoding on FGFs may be examined. If in the existence of a viral vector, the role of the FGFs is to trigger correct growth, then the hypothesis is incorrect, or else the hypothesis is right. The FGF8 is by far the most powerful of all as the result.