Question

The gene being transferred is also cut. Will the restriction enzymes being used to cut the plasmid be used to cut the original DNA?

Answer 1

The answer would be Yes!

Answer 2

Step-by-step explanation:

When a restriction enzyme such as BamHI is used to cut the plasmid, it would cut the circle at one place. The cut would open up the circle in the LacZ gene. This is because gene cloners have placed a piece of DNA that has many restriction enzyme cutting sites within the LacZ gene.