Question

(1.2) Design an experiment to determine the adsorption isotherm of fibrinogen to a polyethylene sample in aqueous buffer at physiological pH and temperature. The PE sample has a flat nonporous surface. Briefly describe key steps of the experiment. (5 points)

(1.3) Now you are given an identical PE sample as in Question 1.2 but the sample has been pre-coated with albumin. Then you use this new sample and perform the same experiment in Question 1.2. Make a sketch to show what you think the adsorption isotherm data of both samples (that is, pristine PE surface and albumin-coated PE surface) would look like and briefly explain how they may differ. (5 points)

(1.4) Compare the two surfaces above. Which surface is more likely to cause blood coagulation? Briefly explain why. (3 points)

Answer 1

The Top THREE MOST ABUNDANT PROTEINS ARE-

1. Albumiins 2. Globulins 3. Fibrinogen

1. Albumins -

Serum albumin, often referred to simply as blood albumin, is an albumin found in vertebrate blood. Human serum albumin is encoded by the ALB gene. Other mammalian forms, such as bovine serum albumin, are chemically similar.

2. Globulins-

The globulins are a family of globular proteins that have higher molecular weights than albumins and are insoluble in pure water but dissolve in dilute salt solutions. Some globulins are produced in the liver, while others are made by the immune system.

3. Fibrinogen-

Fibrinogen (factor I) is a glycoprotein complex that circulates in the blood of vertebrates. During tissue and vascular injury, it is converted enzymatically by thrombin to fibrin and then to a fibrin-based blood clot. Fibrin clots function primarily to occlude blood vessels to stop bleeding. Fibrin also binds and reduces the activity of thrombin.