Question

In a famous experiment, membrane proteins on the surface of mouse and human cells were labeled with green and red fluorescent markers, respectively. The two types of cells were then induced to fuse, forming hybrid cells. Immediately after fusion, green markers could be found on the surface of one half of a hybrid cell, and red markers on the other half. Predict the distribution of the markers after the following experimental conditions and explain your predictions in the context of the fluid-mosaic model of membrane structure.

a) After a 40-minute incubation at 37°C. 3 points
b) After a 40-minute incubation at 15°C instead?

Answer 1

When they are fused, they can create new structures and make one half of the mouse, and one-half of the human lipid and protein components will be merged.

Explanation:

(a) After 40 minutes of overnight incubation, the green and red phosphorescent cells will be spread evenly from around compartment at a temperature of 37, the hydroxyl groups will get more oscillations due to that they will have lipophilic securities for a shorter period of time, and also as a fluid, and these two fluorescent probes will quickly spread.

(b) At 15 ° C, due to lower temperatures, the movements in the lipid chains will be smaller, and thus there will be more hydrophobic bonds between them, which will render them less elastic in existence so that the distribution of both green and red proteins is not rapid as a consequence of the irregular or partial spread of proteins across the cell.