Question

The Lacheinmal protein (LHP) is a hypothetical protein that causes people to smile more often. It is inactive in many chronically unhappy people. The mRNA isolated from a number of different unhappy persons in the same family was found to lack an internal stretch of 173 nucleotides that are present in the LHP mRNA isolated from a control group of generally happy people. The DNA sequences of LHP genes from the happy and unhappy families were determined and compared. They differed by just one nucleotide change, and no nucleotides were deleted from the gene. What can you say about the molecular basis of unhappiness in this family

Answer 1

the questionnaire is incomplete, the corresponding questions are attached and then the answers

1.It can be affirmed that the splicing mechanism is altered when the replacement of a nucleotide occurs, the next thing that happens is that the sequence is altered and the splice that happens between intron-extron is affected, by not recognizing the precise site , will go on to the next one, so consequently the mutant mRNA loses 173 base pairs that allow the protein to function properly

2) we can affirm that the amino acid sequence is the same for happy and unhappy people, however, the alteration will be found in the mRNA reading which will produce the difference by not transmitting the information so that the protein exerts its function