Question

In an isothermal gas chromatography experiment using an ECD detector, 1.69 nmols of nchlorohexane, C6H13Cl, was added as an internal standard to an unknown amount of nchlorodecane, C10H21Cl. The area of the first peak to elute was 32434 units and the area of the second peak to elute was 2022 units. Calculate the amount of chlorodecane in the unknown.

Answer 1

The amount of Chlorodecane in the unknown is 0.105nmols

Step-by-step explanation:

a) Since the GC is in an isothermal state, Chlorohexane C6H13Cl (1.69 nmols) because of its lower boiling point will elute first and Chlorodecane C12H21Cl will elute second.

The area of the first peak corresponding to Chlorohexane is 32434 units.

The area of the second peak corresponding to chlorodecane is 2022 units.

Since the response factor of the compound is not given in question and considering the response factor is same for both the compounds, the answer will be as follow:

1.69 nmols of Chlorohexane gives 32434 units

How much of chlorodecane gives 2022 units

By cross multiplication;

Moles of Chlorodecane = 2022*1.69/32434

=0.105nmols