Question

Calculate the approximate percent increase in luciferase activity between cells containing plasmid pCD3γ-59 and cells containing plasmid pCD3γ-149. Round to the nearest whole number.

Answer 1

The approximate percent increase in luciferase activity is 50%

Why is this correct?

The recorded relative leuciferase activity (RLA) for pCD3\gamma-59 is 2 units (Equation 1), whereas pCD3\gamma-149 demonstrates an RLA of 3 units (Equation 2).

The disparity between these values equates to an increase of 1 unit.

Expressed as a percentage, this increase relative to the RLA of pCD3\gamma-59 can be calculated by using the formula;

% increase = (Increase / RLA of pCD3\gamma-59 ) x 100 = (1/2) x 100

=50%.

See attached images for missing part of the question:

Answer 2

Incomplete question. Here's the full question;

Identify the plasmid that must contain the CD3γ core promoter sequence but the fewest or no negative regulatory sequences. Based on the data in Figure 2, describe the most likely cause of the variation in luciferase activity among the cells that contain plasmids pCD3γ-419 , pCD3γ-309 , pCD3γ-239 , and pCD3γ-199 . Calculate the approximate percent increase in luciferase activity between cells containing plasmid pCD3γ-59 and cells containing plasmid pCD3γ-149 . Round to the nearest whole number.

Step-by-step explanation:

Before answering you'll need the image in figure 2 which provides additional details.