Question

Assume you inoculated 100 cells, with a generation time of 20 minutes, into 100 ml of nutrient broth. You then inoculated 100 cells of the same species into 200 ml of nutrient broth. After incubation for 4 hours, you can reasonably expect to have __________.

A) the same number of cells in both.B) more cells in the 100 ml.C) more cells in the 200 ml.D) The answer cannot be determined based on the information provided.

Answer 1

The correct answer is option A) "the same number of cells in both".

Step-by-step explanation:

The volume of nutrient broth in two different cell cultures is not a variable that would affect the number of cells that are produced. The only way that this could be a factor would be if the cell number is high enough that the nutrients could be depleted. However, in this case where 100 cells with a generation time of 20 minutes are inoculated and the incubation time was of only 4 hours, the volume of nutrient broth is not a factor that would affect them.