Question

C. (1) A new form of Cry1A protein has been developed that targets the mutated cadherin protein and confers resistance to mutant rootworm genotypes. Order the following steps, (1 through 5) to introduce the gene for the new Cry1A protein into an elite corn hybrid. Answer using numbers 1, 2, 3, 4, 5 _____ Test for expression of the gene in transgenic plants _____ Regenerate plants carrying the new gene by tissue culture _____ Create a construct carrying the gene using recombinant DNA technologies _____ Transform the construct carrying the gene into corn plant cells _____ Move the gene into high-performing varieties

Answer 1

1) Create a construct carrying the gene using recombinant DNA technologies.

2) Transform the construct carrying the gene into corn plant cells.

3) Regenerate plants carrying the new gene by tissue culture.

4) Test for expression of the gene in transgenic plants.

5) Move the gene into high-performing varieties by conventional breeding.

Step-by-step explanation: