Question

Maria wanted to Gram stain some bacteria growing on cheese she found in the back of her refrigerator. She couldn't find any crystal violet, but did have crystal yellow. An email to her BIO 1510 lab instructor assured her that the two stains had exactly the same properties. She was able to perform the rest of the procedure according to the usual protocol. When she looked at her slide she found two organisms. One was rod-shaped and yellow in color, the other was pink and spherical. What color would the Gram negative bacteria be if she didn't counterstain the slide

Answer 1

If mordant isn't utilized the both gram positive and negative cells show up green (if natural green utilized)

Clarification here severe utilized is iodine.after the option of essential stain(crystal violet) when we include iodine,it will respond and frame precious stone violet-iodine complex. We realize that gram positive cells have progressively acidic protoplasm.also peptidoglycan layer of gram positive microorganisms is thick, and these properties make them ready to hold the precious stone violet-iodine complex. Furthermore, after decolonization because of this property gram positive microorganisms won't be de-colorized and hold essential stain(pink).

So if severe isn't included gram positive microorganisms the gem iodine complex won't be framed thus precious stone violet alone can't tie emphatically to peptidoglycan and will be de-colorized and produce shade of counter-stain (sufranine-pink,neutral green-green).

Here gram positive is purple and round.