Question

Alpha-mannosidase follows Michaelis-Menten kinetics. A 3 micromolar solution of alpha-mannosidase catalyzes the formation of .66 moles of p nitrophenol is-1 when incubated with the substrate 4-nitrophenyl alpha D-mannoside at a concentration 130 times greater than the Km for that substrate. What is alpha mannosidase Km

Answer 1

220

Step-by-step explanation:

V₀= Vmax(D)/ (D) + km

0.66/100=vmax(130km)/(130km)+(km)

Vmax= 0.66×131/130×1000 =0.6650mm/d

kwat= vmax/ET

=0.665×10⁻³/ 3×10⁻⁶

= 0.22×10³

= 220