Question

What were your predicted results for glucose transport in the presence of DNP? Briefly state the physiologic basis for your prediction. Did your results in lab support your prediction? If not, provide a possible explanation.

Answer 1

Prediction results for glucose transport in the presence of Dinitrophenol (DNP) are as following:

1. Decreased transport of glucose
2. DNP disrupts the gradient of protons and the output of ATP
3. ATP needed for the Na+/K+ pump to the est. Gradient Na+
4. The gradient required for Sodium-glucose cotransporters (SGLTs)

Dinitrophenol (DNP) is an uncoupler, or is capable of separating electron flow and H+ ion pumping for ATP synthesis. This means that ATP synthesis can not use the energy from electron transfer, hence it will support in lab as well.