Question

The spectrophotometric absorbance of three different solutions could be measured in this experiment to determine the concentration ratio of unbound ferrihemoglobin (Hb.Fe3+ ) to bound ferrihemoglobin-imidazole (Hb.Fe3+.Im. These solutions, designated A, A0, and A100 are important. Use these terms to mathematically define: a) [ Hb.Fe3+ ] and b) [Hb.Fe3+.Im]

Answer 1

The meaning f mathematical term is given below

A - This is defined as the absorbance of that solution which have all Bound hemoglobin imidazole comlex.

so according to this [Hb.Fe3+] = 0

[Hb.Fe3+.Im]= 100

concentration can be calculated by lambert beer law

A=
`\epsilon` Cl

where A = absorbance

`\epsilon=` molar absorbance coefficient

l = path length

A0 - This is defined as the absorbance of compeletely unbound hemoglobin . this is for non complex with imidazole.

[Hb.Fe3+.Im]= 0

[Hb.Fe3+]= 100

A100 - this is defined as the absorbance of hemoglobin imidazole complex when all hemoglobin is present in bound state.