Question

A third conditional KO mouse is generated, this time knocking out the activity of lactate dehydrogenase in skeletal muscles. The WT and KO mouse are subjected to 20 minutes of aerobic activity on a running wheel. Immediately after the bout of aerobic activity, metabolite analysis revealed low muscle levels of 1,3-bisphosphoglycerate and phosphoenolpyruvate in the KO mouse compared to the WT mouse. Please explain this finding.

Answer 1

During Glycolysis, G3P gets changed over into 1,3-bisphosphoglycerate and in this way to phosphoenolpyruvate. Transformation of G3P requires NAD+ which is produced by enzymatic activity of lactate dehydrogenase on pyruvate. Lactate dehydrogenase changes over pyruvate to lactate and in the process changes over NADH to NAD+. This NAD in addition to is utilized to change over g3p into 1 3 bisphosphoglycerate. At the point when lactate dehydrogenase is knocked out NAD Plus isn't produced and G3P is not changed over and thus 1, 3 bisphosphoglycerate and PEP isn't created.