Question

• Treatment A: No nuclease treatment.• Treatment B: Brief (30sec) nuclease digestion of naked DNA.• Treatment C: Extended (20min) nuclease digestion of chromatin• Treatment D: Brief (30sec) nuclease digestion of chromatin.Q4.1 Which treatment(s) are the controls? (0.5 points)Treatment a and

Answer 1

Treatment A and treatment B are the controls.

Step-by-step explanation:

Treatment A and treatment B are the controls.

There is no nuclease treatment in treatment A. This gives no DNA fragments and the chromatin is unimpaired.

In treatment B, naked DNA is digested with a nuclease. Since micrococcal nuclease particularly cuts exposed or naked DNA, it cuts this naked DNA into several fragments with different lengths.

Both of these treatments are the controls. Treatment A tells us about the length of the chromatin fiber and what happens when no nuclease treatment is given, while treatment B tells us what happens to the exposed DNA once it is treated with micrococcal nuclease.