Question

A 1.5-kb fragment of DNA is cloned into the EcoRI site of a plasmid vector that is 5.5-kb long. The plasmid vector is then transformed into bacteria. If the plasmid DNA is then extracted from a single bacterial colony and digested with EcoRI, what digestion products will be produced if the plasmid lacks the fragment?

A) one 5.5-kb fragment
B) one 1.5-kb fragment
C) one 7-kb fragment
D)one 1.5-kb fragment

Answer 1

A) ? I would assume this because the plasmid lacks the fragment.