Answer:
The optimal pH can be measured by looking at enzyme activity verses pH. Enzyme activity will increase up to a certain pH; above that pH, the enzyme activity will begin to fall. Graphically, measurement of optimal pH will look like a mountain. The very top indicates the optimal pH.
Step-by-step explanation:
b) First figure out moles of enzyme: 1 mg = 0.001g / 120 kiloDa per mole = 8.3 X 10-6 moles per mg enzyme Then figure out moles of active site, since there are 6 per enzyme. So, 6 X 8.3umoles = 0.05umoles of active sites per mg of pyrophosphatase.
How do you find the initial velocity of an enzyme kinetics from absorbance?
To do this, you calculate the slope of the linear standard curve, which is in units of absorbance change/µM PNp. Divide the initial rate (delta absorbance/min) by the slope of the standard curve (delta absorbance/µM) to get µM/min. This can also be written as µmoles/min/liter, which is also units/L.Enzymes are affected by changes in pH. The most favorable pH value - the point where the enzyme is most active - is known as the optimum pH. ... Extremely high or low pH values generally result in complete loss of activity for most enzymes. pH is also a factor in the stability of enzymes.