Question

1) I have to find the total yield of protein (rGFP) given estimated purity (in %) and amount of proteins. I have the purity of the protein and the amount of protein, so how do I find the total yeild from these values.

2) The expression and purification of rGFP started with the growth of the bacterial culture and ended with elution of rGFP off the Ni+2 agarose coloumn. Describe one change to this process that you predict would result in an increase of the total yield of rGFP.

Answer 1

Please look at the explanation section.

Step-by-step explanation:

1) To calculate the yield of a protein in the purification step, the amount of protein produced must be divided by the amount of protein produced in the first step. The expression is as follows:

%protein yield = (Amount of protein/Amount of protein produced in the first step) * 100

If you have the purity percentage and the amount of protein produced, this formula could be used:

%protein yield = (Protein amount * Purity Percentage)/100

2) If this process begins with a cell culture and ends with the purification of Ni+2NTA. One method of increasing performance is to decrease the number of steps in the purification process. Because unfortunately, at each step of the purification of a protein leads to the loss of them.