Question

Calculate the final concentration of ONPG (in mM) if you add 1.42 mL of 3.3 mM ONPG and dilute to a final volume of 10 mL with PBS buffer. Report your final answer to two places after the decimal.

Answer 1

Answer : The final concentration of ONGP is, 0.47 mM

Explanation :

Formula used :

`M_1V_1=M_2V_2`

where,

`M_1\text{ and }V_1` are the initial molarity and volume

`M_2\text{ and }V_2` are the final molarity and volume

We are given:

`M_1=3.3mM\\V_1=1.42mL\\M_2=?\\V_2=10mL`

Now put all the given values in above equation, we get:

`3.3mM* 1.42mL=M_2* 10mL\\\\M_2=0.47mM`

Hence, the final concentration of ONGP is, 0.47 mM