Question

You are performing an experiment on a cultured animal cell and observing the Golgi complex as part of your study. You add colchicine to the cultured cells and when you do, the elements of the Golgi complex disperse into the cell periphery. When colchicine is removed, the Golgi membranes return to their normal position in the cell interior. What conclusion would you draw from this result

Answer 1

Role of colchicine

Step-by-step explanation:

The correct positioning of the Golgi apparatus in the cell is dependent on intact microtubules

• Golgi complex consists of several tubules and sac like structure present in cytoplasm of all eukaryotic cells
• Microtubules are hollow,inextensible and cylindrical structure which is responsible for anchoring of membrane bound organelles
• During cell division,microtubules disassemble and then reassemble and forms mitotic spindle apparatus and catalyse movement of chromosome
• Colchicine tightly binds with the free tubulin dimer and prevent its polymerization
• In this case mitotic spindle apparatus do not assemble and cell unable to do partitioning of chromosomes into two groups and arrest cell division