Question

A 1.5‑kb fragment of DNA is cloned into a plasmid vector that is 5.5 kb long at the EcoRI site, and the plasmid vector is then used to transform bacteria. If the plasmid DNA is then extracted from a single bacterial colony and digested with EcoRI, what digestion products will be produced if the plasmid contains the fragment?

Answer 1

The answer is One 1.5‑kb fragment and one 5.5‑kb fragment.

Step-by-step explanation:

EcoRI digestion will result into 2 fragment corresponding in size to the 1.5‑kb fragment which will be cloned into the plasmid + the 5.5‑kb plasmid itself.