Answer:
Separation of sister chromatids and centromeres.
Step-by-step explanation:
Once chromosomes align along equatorial plate during metaphase of mitosis, the next step of mitosis is separation of the sister chromatids and the centromeres. The separation of sister chromatids and the centromeres occurs during anaphase of mitosis. During this phase, spindle fibres which are produced from centrioles in the centrosome at two opposite poles of the cell, attach to the centromere region of the chromosomes and drag each sister chromatid of the chromosome apart towards opposite pole of the cell. This is how each opposite pole receives one chromatid from each chromosome. Later during telpophase of mitosis, cytokinesis occurs which produces two daughter cells with equal number of chromatids.