Question

Below are several steps involved in FISH. From the choices available, pick the order of steps that would be successful. 1.Fix chromosomes and denature the DNA., 2.Arrest cells in metaphase., 3.Take a picture using ultraviolet light., 4.Drop cells onto microscope slide to open the cells., 5.Label a DNA probe bound to fluorescent dye., 6.Hybridize the DNA probe to the chromosomes.

Answer 1

the correct order is as follows:

4) Drop cells onto microscope slide to open the cells.

1) Fix chromosomes and denature the DNA

2) Arrest cells in metaphase.

5) Label a DNA probe bound to fluorescent dye.

6) Hybridize the DNA probe to the chromosomes.

3) Take a picture using ultraviolet light.

Step-by-step explanation:

Since the procedure is to be performed on a microscope slide, that's how you start with the procedure. The DNA has to be denatured for the FISH technique to work. The fluorescently labeled DNA probe will bind with the DNA due to complimentary bases and you will be able to view this under ultraviolet light.

Hope that answers the question, have a great day!