Question

Suppose you cut dna you know that you should find four dna fragments on a gel, but only three appear and one fragment is very large. Explain what happen

Answer 1

Restriction Mapping

Step-by-step explanation:

A mutation has occurred and can be known that how many need to be seen by looking at a similar sequences restriction map, indicator of genetic disease

• Restriction mapping is the technique which is used to determine the relative location of restriction sites on a DNA fragment to give a restriction map
• Restriction enzymes recognize specific sequences on DNA and make specific cuts
• Restriction mapping involves the positioning of relative location of restriction sites on a DNA fragment
• Some enzymes require four bases as a restriction sites and make frequent cuts and generate small fragments while some enzymes recognize six bases or eight bases and make rare cuts to generate large fragments
• DNA fragments after the digestion are separated in agarose gel
• In the given question one fragment comes out to be very large,it is possible that mutation might have occurred and which can be observed by looking at other restriction map of similar sequences