Answer:
the Sox2 protein is a prime factor of making neutral tissues and has an important role in, initiating neural induction and maintaining neural progenitor stem cell properties throughout neural differentiation.
a researcher removed a group of cells believing that it was the neural specified area and placed them in a neutral culture medium. he did not observe and those cells did not appear as a neuron-like appearance because--
The removed cells needed to be exposed to a variety of "non-neural" proteins to demonstrate neural specification.
it was left unattended and non-neural proteins were also not been provided to that culture.