Question

how would you prepare an absportion spectrum for a purified soltion of b-carotene dissolved in acetone using the materials and equipment outlined in the lab manual

Answer 1

Use a series dilution

Step-by-step explanation:

The rationale behind the experiment is that the different substances absorb light at different wavelengths. To prepare an absorption spectrum for a purified solution of b-carotene dissolved in acetone, firstly, the
`\beta` -carotene needs to be diluted enough to reach the light spec in the 20 tube. This will be done through a series of serial dilutions of a known concentration of the chlorophyll component. The bankmill will consist of acetone only and 1 blank will be needed. The range of the wavelength would be in the range of 400 - 700 nm. So, the intervals of 20 nm will suffice. Then, the OD for each wavelength will be used to determine the adsorption.