Question

Each of the following statements describes a type of column chromatography. Sort the statements to the type of chromatography they describe. If a statement can describe all of the types, place that statement in the "All" category. (Note that size-exclusion chromatography may also be called gel-filtration or molecular exclusion chromatography.)Categories: Size-exclusion Chromatography, Affinity Chromatography, Ion-exchange Chromatogrpahy, and AllStatements:Separate molecules by size.Separate molecules by charge.The stationary phase has a covalently bound group to which a protein in the mobile phase can bind.Uses a mobile phase and a stationary phase to separate proteins.The stationary phase contains cross-linked polymers with different pore sizes.Can separate molecules based on protein-lignand binding.The stationary phase may contain negatively or positively charged groups.

Answer 1

ALL: it can all be used in the Separation of molecules by size.

ION-EXCHANGE CHROMATOGRAPHY, AFFINITY CHROMATOGRAPHY: are the type if chromatography used to Separate molecules by charge.

ALL: each given separation technique as its stationary phase notably possessing a covalently bound group where a protein in its mobile phase can adhere to.

ALL: it all employs a mobile phase and a stationary phase to distinguish between proteins.

SIZE-EXCLUSION CHROMATOGRAPHY: is stationary phase it's know to have cross-linked polymers which possess several pore sizes.

AFFINITY CHROMATOGRAPHY: this is used majorly to distinguish between molecules using their protein-ligand binding.

ION-EXCHANGE CHROMATOGRAPHY, AFFINITY CHROMATOGRAPHY: both are known to have a stationary phase that make use of a negatively or positively charged groups.