Question

You are trying to match DNA found at a crime scene with two possible suspects. Why would it be a better use of your time and resources to analyze the DNA using a double restriction digest rather than a single digest

Answer 1

A double restriction digest is better than a single digest when analyzing DNA at a crime scene with possible suspects. It provides more specific and accurate results, reducing the chances of false matches. It also provides additional information about the DNA, aiding in identifying specific genetic markers or variations.

Step-by-step explanation:

When trying to match DNA found at a crime scene with possible suspects, it is better to analyze the DNA using a double restriction digest rather than a single digest for a few reasons.

Firstly, a double restriction digest provides more specific and accurate results. By using two different restriction enzymes, you obtain two different sets of fragment DNA. This allows for a more precise comparison of the DNA banding patterns between the crime scene DNA and the suspects' DNA. It helps to reduce the chances of false matches or inconclusive results.

Secondly, a double restriction digest provides additional information about the DNA. By analyzing the fragment sizes produced by two different enzymes, you can gather more information about the genetic makeup of the DNA. This can be useful in identifying specific genetic markers or variations that can further support or rule out the involvement of a suspect.

Overall, a double restriction digest is a more thorough and reliable method for DNA analysis in forensic investigations, as it provides more specific results and additional genetic information.

Answer 2

Answer: A double restriction digest

Explanation: A double restriction digest is a process were two restriction enzymes are used to digest DNA in a single reaction.in double restriction digest you will observe two bands,while in single restriction digest you will observe just one band. In double restriction digest no

Self - ligating plasmid because it create mismatch ends. It also aids in directional in section while single disgestion you have to screen your clones to be sure to get your plasmid with correct insert orientation.