Question

Using a spectrophotometer, and a cuvette with a path length of 1 cm you measure the absorbance (A275) of Guanosine to be 0.70. Calculate the concentration of guanosine in your sample

Answer 1

Answer : The concentration of guanosine in your sample is,
`8.33* 10^(-5)M`

Explanation :

Using Beer-Lambert's law :

`A=\epsilon * C* l`

where,

A = absorbance of solution = 0.70

C = concentration of solution = ?

l = path length = 1.00 cm

`\epsilon` = molar absorptivity coefficient guanosine =
`8400M^(-1)cm^(-1)`

Now put all the given values in the above formula, we get:

`0.70=8400M^(-1)cm^(-1)* C* 1.00cm`

`C=8.33* 10^(-5)M`

Thus, the concentration of guanosine in your sample is,
`8.33* 10^(-5)M`