Answer:
In a TLC experiment, if no spots are seen then it might be due to the following reasons:
- It might be that the concentration of the sample might be too low. This problem can be overcome by spotting the sample multiple times.
- There might be a problem with the development method and we shall look for alternative methods.
- It might also happen that there might be no compound in your sample due to errors in the experiment performed. You should have a re-check of your experiment.
- The level of the solvent system might be too high. Re-conduct the experiment with right amount of solvent.