Question

You use the Bradford (coomassie) assay to determine the concentration of a whey protein. You use bovine albumin as your standard. You determine the histone protein concentration to be 7 mg/ml.When you use the same histone protein as your standard, you determine the histone protein concentration to be 5 mg/ml. Explain the discrepancy (pick ONE answer): (2 points)a. The histone protein has fewer basic amino acids than albumin.b. The histone protein has more acidic amino acids than albuminc. The histone protein has more basic amino acids than albumind. The histone protein contains more tryptophan than albumin\

Answer 1

Answer: A: the histone protein has fewer basic amino acids than albumin

Step-by-step explanation:

The histones are know to be less basic than the protamines. High amounts are contain in either lysine or arginine while aspartic acid and glutamic acid are in smaller. Histones occur in combination with DNA as nucleohistones in the nuclei of the body cells of plants and animals, but it is not present in the sperm of an animal . The molecular weights of histones vary from 10,000 to 22,000. In contrast to the protamines, the histones contain most of the 20 amino acids, with the exception of tryptophan and the sulfur-containing ones. Just like the protamines, histone preparations are heterogeneous mixtures. The sequence of The histones are less basic than the protamines. They contain high amounts of either lysine or arginine and small amounts of aspartic acid and glutamic acid. Histones occur in combination with DNA as nucleohistones in the nuclei of the body cells of animals and plants, but not in animal sperm. The molecular weights of histones vary from 10,000 to 22,000. In contrast to the protamines, the histones contain most of the 20 amino acids, with the exception of tryptophan and the sulfur-containing ones. Like the protamines, histone prepara

histone preparations are heterogeneous mixtures. The sequence of the amino acid of some of the histones has been determined.