Question

Describe the interactions between molecules in a mixture (analyte), the stationary phase and various mobile phases used in column chromatography and the effect of these interactions on the separation of the mixture.

Answer 1

Hydrophobic interactions.

Step-by-step explanation:

• Column chromatography is a technique used for separation in which a mixture having a mobile phase is caused to move in contact with the stationary phase.
• The Stationary phase used is a solid and the mobile phase used is liquid.

• There are interactions between the molecules of the analyte, stationary phase, and the various mobile phases like hydrogen bonding and dipole-dipole interactions.

• The effect of these interactions is that the compound is retained for a longer time and as there are different interactions between stationary and mobile phases they are carried along with the mobile phases with varying degrees and the separation of the mixture is done.